New
New
Year 7

Preparing and observing a microscope slide

I can prepare a microscope slide with a specimen of tissue, observe it using a microscope, and record my observations in a scientific line drawing.

New
New
Year 7

Preparing and observing a microscope slide

I can prepare a microscope slide with a specimen of tissue, observe it using a microscope, and record my observations in a scientific line drawing.

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Lesson details

Key learning points

  1. The cells that make up tissue can be viewed using a light microscope.
  2. A sample of tissue can be prepared on a slide to view using a microscope.
  3. Adding a stain can make it easier to see some parts of cells.
  4. Changing the magnification and focus of a light microscope enables stained cell structures to be observed.
  5. Observations from a light microscope can be recorded in a scientific line drawing.

Keywords

  • Tissue - A tissue is a group of similar cells with the same job working together.

  • Slide - To observe a specimen using a light microscope, we have to put a thin layer of it on a glass microscope slide.

  • Stain - A coloured liquid put onto a specimen so that the cells and their structures can be more easily seen with a light microscope.

  • Scientific line drawing - Observations from a light microscope can be recorded by making a labelled scientific line drawing.

Common misconception

Producing a line drawing with sketched broken lines, shading, no labelling and no magnification.

Learning cycle 2 in this lesson explores how to produce a scientific line drawing to record observations from light microscopy.

Pupils should be provided with the opportunity to produce their own line drawings to record observations from light microscopy, and perhaps to peer review them in a constructive way.
Teacher tip

Content guidance

  • Risk assessment required - equipment

Supervision

Adult supervision required

Licence

This content is © Oak National Academy Limited (2024), licensed on Open Government Licence version 3.0 except where otherwise stated. See Oak's terms & conditions (Collection 2).

Lesson video

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6 Questions

Q1.
A is a piece of equipment that is used to see things that are very small.
Correct Answer: microscope, light microscope
Q2.
Which parts of the microscope magnify an image?
Correct answer: eyepiece lens
light
focusing wheel
Correct answer: objective lens
Q3.
Which is not a part of a microscope?
focusing wheel
light/mirror
Correct answer: telescope
stage
eyepiece lens
Q4.
Which action would you take to increase the magnification of this image (to make the individual cells appear larger)?
An image in a quiz
move the stage
increase the brightness of the light
turn the focusing wheel
Correct answer: increase the value of the objective lens
Q5.
Which of these images is more focused?
An image in a quiz
Correct Answer: An image in a quiz
An image in a quiz
Q6.
A is a piece of curved glass that focuses and magnifies light.
Correct Answer: lens

6 Questions

Q1.
Which piece of apparatus is not used to prepare an onion tissue slide?
mounted needle
tweezers
cover slip
Correct answer: magnifying glass
Q2.
Match the apparatus to its job in preparing a microscope slide.
Correct Answer:glass slide,put the specimen on

put the specimen on

Correct Answer:stain,to see the specimen more clearly

to see the specimen more clearly

Correct Answer:cover slip,to place over the specimen

to place over the specimen

Correct Answer:tweezers,to hold the tissue and cover slip

to hold the tissue and cover slip

Correct Answer:mounted needle,to lower the cover slip slowly

to lower the cover slip slowly

Q3.
Starting with a glass slide, put these stages in the correct order for preparing a microscope slide.
An image in a quiz
1 - Take a glass slide and place a tissue or cell specimen on it.
2 - Add a drop of stain.
3 - Place a cover slip over the top using tweezers.
4 - Lower the cover slip with a mounted needle.
5 - Blot off excess stain.
Q4.
The chemical that is placed on a specimen so that it can been seen easily is called a...
Correct Answer: stain
Q5.
Which features should be altered in this scientific line drawing to improve it?
An image in a quiz
Correct answer: sketched lines
using pencil
cell shape
Correct answer: shading
Q6.
Which of these descriptions is not a key feature of an accurate scientific line drawing?
An image in a quiz
labelling
continuous, smooth lines
stippling
Correct answer: shading
magnification used
Q3 UYSEG Q5 UYSEG Q6 UYSEG

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