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      The effect of light on water uptake by a plant: practical

      Lesson details

      Learning outcome

      I can use a simple potometer to investigate the effect of light on water uptake by a plant.

      Key learning points

      1. Practical demonstration of the use of a simple potometer to measure water uptake by a leafy shoot.
      2. Use of a light source to vary light intensity.
      3. Use of appropriate apparatus to control temperature (e.g. a water tank as a heat shield between light and plant).
      4. Use of a simple potometer to measure water uptake by a plant at a range of light intensities.
      5. Rate of water uptake = change in volume of water ÷ time taken for volume to change

      Keywords

      • Transpiration - the loss of water from a plant’s leaves

      • Potometer - a piece of equipment used to measure the rate of water uptake by a plant

      • Control - a variable which is kept constant between all experiments

      • Light intensity - the amount of light reaching a given area in a given time

      • Rate - a measure of how much change occurs per unit of time

      Common misconception

      Being able to explain the set up of a potometer is difficult because it has so many stages that are tricky.

      The stages of setting up a potometer are broken down into four parts, checked for understanding, reinforced with a lab demonstration video and concluded with a cartoon task.

      Teacher tip

      Demonstrate the set up of a potometer, and perhaps if you have time, allow students to simulate parts or all of the set up process. Running the practical is optional, as it takes a while and can yield poor quality data.

      Equipment

      Optional: potometer, leafy shoot, clamps, clamp stand, large beaker, tripod, gauze, lamp, metre rule, timer.

      Content guidance

      Risk assessment required - equipment

      Supervision

      Adult supervision required

      Licence

      This content is © Oak National Academy Limited (2026), licensed on Open Government Licence version 3.0
      except where otherwise stated. See Oak's terms & conditions
      (Collection 2).

      Lesson video

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      Prior knowledge starter quiz

      6 Questions

      Q1.
      What do plants need to perform photosynthesis?

      carbon dioxide, oxygen, and water
      oxygen, light, and glucose
      Correct answer: water, light, and carbon dioxide
      water, oxygen, and sunlight

      Q2.
      Which part of the plant is primarily responsible for water absorption?

      flowers
      leaves
      Correct answer: roots
      stems

      Q3.
      What is the function of stomata in leaves?

      Correct answer: gas exchange and water loss
      nutrient absorption
      photosynthesis
      water uptake

      Q4.
      What is transpiration?

      the absorption of nutrients by roots
      the exchange of gases in leaves
      the process of photosynthesis
      Correct answer: the process of water loss from the leaves

      Q5.
      What is the transportation stream?

      the flow of water in a river
      the evaporation of water from the leaf of a plant
      Correct answer: the movement of water and dissolved minerals through a plant

      Q6.
      How do you calculate the rate of a process?

      Correct answer: change ÷ time taken
      change × time taken
      time taken ÷ change

      6 Questions

      Q1.
      What does a potometer measure?

      the rate of gas exchange
      the rate of photosynthesis
      the rate of transpiration
      Correct answer: the rate of water uptake by a plant

      Q2.
      How does light intensity affect the rate of water uptake in plants?

      Higher light intensity decreases the rate of water uptake.
      Correct answer: Higher light intensity increases the rate of water uptake.
      Light intensity has no effect on water uptake.
      Lower light intensity increases the rate of water uptake.

      Q3.
      What is the formula to calculate the rate of water uptake?

      Correct answer: change in volume of water ÷ time taken
      change in volume of water × time taken
      volume taken ÷ time change
      time taken ÷ change in volume of water

      Q4.
      Why is a beaker of water used in this experiment?

      An image in a quiz
      Correct answer: to control temperature
      to increase light intensity
      to measure light intensity
      to protect the plant from water loss

      Q5.
      What are the roles of stomata in leaves?

      Correct answer: gas exchange
      nutrient absorption
      photosynthesis
      Correct answer: to control water loss

      Q6.
      During a potometer experiment lasting 20 minutes, the start reading was 0.5 cm³ and the end reading was 3.2 cm³. What is the rate of water uptake?

      Correct answer: 0.135 cm³/min
      0.105 cm³/min
      0.145 cm³/min
      0.120 cm³/min

      To help you plan your 11 biology lesson on: The effect of light on water uptake by a plant: practical, download all teaching resources for free and adapt to suit your pupils' needs...