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      Lesson details

      Learning outcome

      I can carry out tests for biological molecules, including sugars, starch, proteins and lipids.

      Key learning points

      1. A reagent called Benedict’s solution is used to test for reducing sugars, including glucose.
      2. A reagent called iodine solution is used to test for starch, a type of carbohydrate.
      3. A reagent called Biuret solution is used to test for protein.
      4. Ethanol (a type of alcohol) is used to test for lipids.
      5. Interpretation of qualitative food test results.

      Keywords

      • Reagent - A chemical used to test for a type of biological molecule.

      • Qualitative - A test in which the result is based on an observation.

      • Quantitative - A test in which a measurement produces numerical data.

      Common misconception

      Mistakes on method, results confusion, and poor descriptions using inaccurate terminology.

      Methods and results are described, demonstrated and tested throughout, and methods tried by pupils with results gathered and analysed. Repeated review of results occurs throughout the lesson, and methods and results are applied to novel questions.

      Teacher tip

      Allow the pupils to complete these practicals by themselves. Allow them to use coloured pencils to illustrate their note taking, and consider memory hooks like 'purple protein' to remember the test and its results.

      Equipment

      Distilled water, Benedict's solution, Biuret's solution, sodium hydroxide, iodine solution, ethanol, test/boiling tubes, bung, pipettes, 60ºC water bath, safety goggles.

      Content guidance

      Risk assessment required - equipment

      Supervision

      Adult supervision required

      Licence

      This content is © Oak National Academy Limited (2026), licensed on Open Government Licence version 3.0
      except where otherwise stated. See Oak's terms & conditions
      (Collection 2).

      Lesson video

      Loading...

      Prior knowledge starter quiz

      6 Questions

      Q1.
      True or false? Proteins and carbohydrates are polymers.

      Correct answer: true
      false

      Q2.
      Join the polymer to its monomer.

      Correct Answer:protein,amino acids

      amino acids

      Correct Answer:carbohydrates,sugar

      sugar

      Q3.
      True or false? Enzymes are proteins.

      Correct answer: true
      false

      Q4.
      Which of these is a use for lipids in an organism?

      making cell walls
      making cellulose
      Correct answer: making cell membranes
      making enzymes
      making energy

      Q5.
      True or false? Animals store carbohydrate as glycogen.

      Correct answer: true
      false

      Q6.
      What elements do carbohydrates contain?

      An image in a quiz
      water
      Correct answer: carbon
      Correct answer: oxygen
      nitrogen
      Correct answer: hydrogen

      6 Questions

      Q1.
      Match the reagent to the nutrient it us used to test for?

      Correct Answer:iodine,starch

      starch

      Correct Answer:Benedict's solution,reducing sugar

      reducing sugar

      Correct Answer:Biuret solution,protein

      protein

      Q2.
      What colour does iodine turn in the presence of starch?

      Correct answer: black
      orange
      yellow
      green
      violet

      Q3.
      What substance can be used to test for the presence of lipids?

      Correct Answer: ethanol, alcohol

      Q4.
      Is this a positive or a negative test for Benedict's solution?

      An image in a quiz
      Correct answer: positive
      negative

      Q5.
      What colour does Biuret solution turn if it comes into contact with protein?

      Correct Answer: Purple, Violet, Light purple

      Q6.
      True or false? This food contains a small amount of reducing sugar?

      An image in a quiz
      true
      Correct answer: false

      To help you plan your 10 combined science lesson on: Tests for biological molecules, download all teaching resources for free and adapt to suit your pupils' needs...