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      The effect of pH on the rate of an enzyme reaction: plan

      Lesson details

      Learning outcome

      I can write a testable prediction and plan an investigation into the effect of changing pH on the rate of an enzyme reaction.

      Key learning points

      1. Planning an investigation into the effect of pH on the rate at which amylase enzyme breaks down starch into maltose
      2. Writing an aim for the investigation, and a testable prediction
      3. Identifying independent and dependent variables, and which factors to control
      4. Planning a method, to include use of iodine in continuous sampling to indicate when all starch has been broken down
      5. Identifying hazards and risks, and how to limit them

      Keywords

      • PH - A measure of how acidic or alkaline a solution is.

      • Rate of reaction - How fast a chemical reaction occurs.

      • Starch - A carbohydrate, which is a polysaccharide (a polymer of sugar).

      • Amylase - An enzyme that breaks down starch into maltose (a type of sugar).

      • Continuous sampling - A method that involves taking regular and frequent samples.

      Common misconception

      Misconceptions mixing aim and prediction or leaving them vague, writing a method which is unclear, and analysing hazards and their risks.

      Approaches for stating aims and predictions, writing a method and dealing with variables, and analysing hazards and their risk are all covered with examples and practice tasks.

      Teacher tip

      Use real practical equipment to demonstrate the methodology of continuous sampling, and to help pupils analyse risk effectively. Ask pupils to handle the equipment and consider how they might use it, so they can approach the tasks with workable ideas.

      Licence

      This content is © Oak National Academy Limited (2026), licensed on Open Government Licence version 3.0
      except where otherwise stated. See Oak's terms & conditions
      (Collection 2).

      Lesson video

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      Prior knowledge starter quiz

      6 Questions

      Q1.
      Which of the following is starch made from?

      amino acids
      Correct answer: sugars
      fatty acids
      glycerol

      Q2.
      Which of the following is an enzyme?

      hydrochloric acid
      Correct answer: amylase
      bile
      saliva

      Q3.
      Which of the following can the pH of a substance tell us?

      Correct answer: How acidic a substance is.
      The temperature of a substance.
      Correct answer: How alkaline a substance is.
      The amount of energy a substance has.

      Q4.
      What substance does iodine test for the presence of?

      Correct Answer: starch

      Q5.
      Which of the following can be used to calculate the rate of a reaction?

      Correct answer: time
      amount of reactants
      Correct answer: amount of product formed
      temperature
      concentration of reactants

      Q6.
      Which of the following describes the meaning of continuous sampling in the context of an experiment?

      Readings are taken at the start and end of an experiment.
      A reading is taken at the end of an experiment.
      A reading is taken at the start of an experiment.
      Correct answer: Readings are taken at regular intervals throughout an experiment.

      6 Questions

      Q1.
      What does the enzyme amylase break down starch into?

      glucose
      Correct answer: maltose
      fructose
      galactose

      Q2.
      A pupil investigates the effect of pH on the rate of amylase activity as it digests starch into maltose. Which of the following is the independent variable in this investigation?

      type of enzyme
      rate of amylase activity
      Correct answer: pH
      amount of starch digested
      amount of maltose

      Q3.
      Match the key words to their definitions.

      Correct Answer:hazard,something that may cause harm

      something that may cause harm

      Correct Answer:risk,the harm that may be caused

      the harm that may be caused

      Correct Answer:limitation,an action taken to reduce the potential for harm

      an action taken to reduce the potential for harm

      Q4.
      A is a statement of what we expect to happen in an experiment

      Correct Answer: prediction

      Q5.
      When testing a food sample for the presence of starch, how do you know when all of the starch has been broken down?

      An image in a quiz
      The colour of the iodine solution changes to blue–black.
      The colour of the iodine solution changes from black to blue.
      The colour of the iodine solution changes from blue to black.
      Correct answer: The colour of the iodine solution does not change to blue–black.

      Q6.
      A pupil investigates the effect of pH on the rate of amylase activity as it digests starch into maltose. Which of the following are control variables in this investigation?

      pH
      Correct answer: temperature
      rate of amylase activity
      Correct answer: amount of starch
      Correct answer: amount of amylase

      To help you plan your 10 combined science lesson on: The effect of pH on the rate of an enzyme reaction: plan, download all teaching resources for free and adapt to suit your pupils' needs...