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      Lesson details

      Learning outcome

      I can explain the effects of limiting factors on the rate of photosynthesis.

      Key learning points

      1. Rate of photosynthesis increases as CO₂ concentration, temperature and light intensity increase from low levels.
      2. The rate of photosynthesis can be limited by one of these factors even if the others continue to increase.
      3. The rate of photosynthesis increases until one of more factors becomes limiting, then does not increase further.
      4. Interpretation and explanation of graphs depicting effect of interacting limiting factors on the rate of photosynthesis.

      Keywords

      • Photosynthesis - The process used by producers to make their own food via a series of chemical reactions.

      • Rate - A measure of how much change occurs per unit of time.

      • Limiting factor - A condition that, when in short supply, slows down or limits the rate of photosynthesis.

      • Enzyme - A biological catalyst that speeds up a chemical reaction without itself being used up.

      • Optimum - The conditions where maximum rate of reaction occurs.

      Common misconception

      Failing to realise that the plateau on a rate graph can occur because another factor (not named on the axes) has become limiting.

      The lesson explores rate graphs in which multiple limiting factors are affecting the rate of photosynthesis.

      Teacher tip

      Pupils can be given graphs containing multiple limiting factors to interpret for themselves or they could be given a set of different conditions and asked to sketch graphs of what they would expect the rate of photosynthesis to be in each scenario, explaining why they have drawn the graph they have.

      Licence

      This content is © Oak National Academy Limited (2026), licensed on Open Government Licence version 3.0
      except where otherwise stated. See Oak's terms & conditions
      (Collection 2).

      Lesson video

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      Prior knowledge starter quiz

      6 Questions

      Q1.
      What is the main function of chloroplasts in plant cells?

      Correct answer: to carry out photosynthesis
      to provide structural support
      to store genetic information
      to transport water

      Q2.
      Which gas is essential for photosynthesis?

      Correct answer: carbon dioxide (CO₂)
      hydrogen (H₂)
      nitrogen (N₂)
      oxygen (O₂)

      Q3.
      What is the chemical formula for glucose?

      C₁₂H₂₂O₁₁
      Correct answer: C₆H₁₂O₆
      CO₂
      H₂O

      Q4.
      Which part of the plant leaf contains the most chloroplasts?

      the cuticle
      Correct answer: the palisade mesophyll
      the spongy mesophyll
      the stomata

      Q5.
      How does carbon dioxide enter the plant leaves?

      through the cuticle
      through the roots
      Correct answer: through the stomata
      through the veins

      Q6.
      What is the main product of photosynthesis?

      carbon dioxide
      Correct answer: glucose
      oxygen
      water

      6 Questions

      Q1.
      What does it mean when a factor is described as a limiting factor?

      it enhances the rate of photosynthesis when in short supply
      it has no effect on photosynthesis
      Correct answer: it slows down or limits the rate of photosynthesis when in short supply
      it stops the process of photosynthesis

      Q2.
      Which of the following are not limiting factors of photosynthesis?

      Correct answer: oxygen concentration
      carbon dioxide concentration
      light intensity
      chlorophyll availability

      Q3.
      What occurs when light intensity reaches optimum levels in photosynthesis?

      An image in a quiz
      the rate of photosynthesis decreases
      photosynthesis stops completely
      Correct answer: the rate of photosynthesis no longer increases
      the rate of photosynthesis increases indefinitely

      Q4.
      What is the relationship between temperature and the rate of photosynthesis?

      An image in a quiz
      temperature has no effect on the rate of photosynthesis
      the rate decreases with temperature
      Correct answer: the rate increases with temperature until enzymes denature
      the rate remains constant with temperature changes

      Q5.
      Which of the following is most likely to be limiting the rate of photosynthesis at point A of the graph?

      An image in a quiz
      light intensity
      Correct answer: carbon dioxide concentration
      oxygen concentration
      temperature

      Q6.
      Which of the following can be concluded from this graph?

      An image in a quiz
      Correct answer: the rate is limited by CO₂ concentration
      the rate is limited by light intensity
      Correct answer: the rate is limited by temperature
      Correct answer: CO₂ concentration has a greater effect on the rate than temperature
      the rate is unaffected by CO₂ concentration

      To help you plan your 11 combined science lesson on: Photosynthesis and limiting factors, download all teaching resources for free and adapt to suit your pupils' needs...