Explaining effects of substrate concentration and temperature on enzyme rate
I can explain how and why the rate of an enzyme reaction is affected by substrate concentration and temperature.
Explaining effects of substrate concentration and temperature on enzyme rate
I can explain how and why the rate of an enzyme reaction is affected by substrate concentration and temperature.
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Lesson details
Key learning points
- The rate of a reaction is the amount of change (e.g. substrate broken down or product made) per unit of time.
- Enzyme reaction rate increases as substrate concentration increases, until an optimum when all active sites are full.
- Enzyme reaction rate increases with temperature due to more collisions, until an optimum when all active sites are full.
- Enzyme rate decreases as temperature increases above the optimum as bonds break and the active site becomes denatured.
- Interpretation of graphs showing effects of substrate concentration and temperature on enzyme reaction rate.
Keywords
Rate of reaction - A measure of the amount of change that occurs per unit of time.
Concentration - A measure of the quantity of a dissolved substance in a given volume of solution.
Optimum - The conditions under which the maximum rate of reaction occurs.
Bond - A force of attraction between atoms in a compound.
Denatured - A permanent change in the shape of an enzyme that stops it from working properly.
Common misconception
Enzymes die rather than denature.
The word denature is clearly introduced and a CfU explores the word die vs denature.
Equipment
Content guidance
- Risk assessment required - equipment
Supervision
Adult supervision required
Licence
Lesson video
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Prior knowledge starter quiz
6 Questions
Q1.What region on an enzyme does the substrate bind to?
Q2.Put the steps in order to show how an enzyme works.
Q3.Enzymes the amount of activation energy needed for a reaction to take place.
Q4.The shape of the active site is to the substrate.
Q5.Which of the following are enzymes?
Q6.What type of molecule are enzymes?
Assessment exit quiz
6 Questions
Q1.Which part of an enzyme does the substrate need to collide with for a reaction to take place?
Q2.What is the rate of reaction?
Q3.Below are two graphs showing the rates of enzyme facilitated reactions. Match the graph to the factor affecting the rate of reaction.
graph B
graph A
Q4.Put these steps in order to show what happens as the temperature of an enzyme controlled reaction increases.
Q5.Where on the graph is the substrate concentration greater than the enzyme concentration?
Q6.Where on the graph could the enzyme concentration be greater than substrate concentration?
