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      Observing mitosis in plant cells using a light microscope (including PMAT)

      Lesson details

      Learning outcome

      You can use a light microscope to observe and identify plant cells in different stages of mitosis.

      Key learning points

      1. A light microscope can be used to observe plant cells in different stages of mitosis (e.g. from onion root tip).
      2. The parts of a light microscope and their functions.
      3. The sequence of steps to set up a light microscope to observe cells, including changing the magnification and focus.
      4. Explaining observations from microscopy using ideas about what happens during the cell cycle (including PMAT).

      Keywords

      • Light microscope - A type of microscope that uses visible light and a system of lenses to generate magnified images of small objects.

      • Mitosis - A type of cell division that produces genetically identical cells.

      • Lens - A piece of glass or other transparent material with curved sides, used in a microscope to magnify objects.

      • Magnification - Making small objects appear larger in order to see more detail.

      • Focus - A point where light rays converge to form an image with clarity.

      Common misconception

      Recognising the difference between images of interphase and prophase. In particular, the idea of DNA condensing to form visible chromosomes during prophase.

      There are two slides that aim to show the difference between DNA held within the nucleus and visible chromosomes where DNA has condensed. There is also a check for understanding question and practise task question to reinforce this concept.

      Teacher tip

      Pupils could use the microscopes to identify the stages of mitosis themselves.

      Equipment

      Light microscopes and prepared slides.

      Licence

      This content is © Oak National Academy Limited (2026), licensed on Open Government Licence version 3.0
      except where otherwise stated. See Oak's terms & conditions
      (Collection 2).

      Lesson video

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      Prior knowledge starter quiz

      6 Questions

      Q1.
      What word describes making objects appear larger using a lens?

      focus
      Correct answer: magnification
      resolution

      Q2.
      What are the smallest living units of life?

      tissues
      organs
      virus
      Correct answer: cells

      Q3.
      What is this piece of scientific equipment called?

      An image in a quiz
      Correct Answer: miroscope, light microscope

      Q4.
      What is mitosis?

      A type of cell division that creates four different cells.
      The process by which the sex cells are formed.
      Correct answer: A type of cell division that creates two genetically identical cells.

      Q5.
      Put these stages of mitosis into the correct order

      1 - interphase
      2 - prophase
      3 - metaphase
      4 - anaphase
      5 - telophase
      6 - cytokinesis

      Q6.
      Which of the following is not true for mitosis?

      Correct answer: Daughter cells have half the amount of DNA.
      Two genetically identical cells are produced.
      Multicellular organisms use mitosis for growth and repair.

      6 Questions

      Q1.
      Which part of the microscope is the line pointing towards?

      An image in a quiz
      eyepiece lens
      coarse focus wheel
      Correct answer: objective lens
      stage

      Q2.
      In which two places does mitosis take place in an onion?

      Correct answer: tips of shoots
      outer layer of skin
      Correct answer: tips of roots
      inner layer of skin

      Q3.
      Put these stages in order to show how a microscope should be used?

      1 - Turn the objective lens to the lowest power.
      2 - Clip the specimen onto the stage and turn on the light.
      3 - Use the coarse focus wheel to roughly focus the image.
      4 - Use the fine focus wheel to get a sharp focus.
      5 - Turn the objective lens to a higher power to increase the magnification.

      Q4.
      The image has circled a stage of mitosis, what stage is this?

      An image in a quiz
      prophase
      Correct answer: metaphase
      telophase
      anaphase

      Q5.
      The image has circled a stage of the cell cycle, what stage is this?

      An image in a quiz
      prophase
      cytokinesis
      telophase
      anaphase
      Correct answer: interphase

      Q6.
      Match the stage of mitosis to the correct description

      Correct Answer:interphase,the DNA and sub-cellular structures are copied.

      the DNA and sub-cellular structures are copied.

      Correct Answer:prophase,nuclear membrane breaks down & DNA condenses to visible chromosomes.

      nuclear membrane breaks down & DNA condenses to visible chromosomes.

      Correct Answer:metaphase,where the chromosomes line up along the equator of the cell.

      where the chromosomes line up along the equator of the cell.

      Correct Answer:anaphase,chromosomes split to form chromatids that are pulled to either side.

      chromosomes split to form chromatids that are pulled to either side.

      Correct Answer:telophase,nuclear membrane re-forms; chromosomes decondense back to DNA strands.

      nuclear membrane re-forms; chromosomes decondense back to DNA strands.

      Correct Answer:cytokinesis,cell membrane & cytoplasm divide forming 2 genetically identical cells

      cell membrane & cytoplasm divide forming 2 genetically identical cells


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